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December 10, 2009

Mini microscope mounted on mices heads can help study human diseases

Stanford University researchers have created a microscope that is small enough to be mounted to the head of a freely moving mouse to watch brain cell activity, and whole animal behaviour simultaneously.
The researchers say that their tiny microscope offers a new way to study human diseases using transgenic mice.
Project leader Mark Schnitzer says that the device weighs just 1.1 grams, and thus can be worn by a mouse without significantly impairing its movement.
He has revealed that his team has already used the device to study the circulation of blood through the one-cell-wide capillaries in the brain of active mice.
The researcher says that the microscope is attached to the head of a mouse under anaesthetic, while a marker dye is injected into the brain to label blood plasma, but leave blood cells unaffected.
According to him, the device uses light delivered by a mercury arc lamp through a bundle of optical fibres, which causes the dyed blood plasma to fluoresce, showing up individual blood cells as dark spots.
The image is sent back up the fibre-optic bundle to a camera that records the image, he adds.
Schnitzer says that nearly 100 images can be taken every second, something that makes it possible for the researchers to watch high-speed video of individual blood cells flowing in the brain.
Once the mouse wakes up from the anaesthetic, according to him, it is possible to watch the movement of cells as it behaves normally.
The researchers have revealed that combining the technique with a dye that makes the activity of brain cells visible, they could see how Purkinje neurons, involved in controlling movement, become more active when a mouse is moving than when resting.

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Source: Thaindian News